花药培养作为一种新的育种手段可缩短育种世代,提高选择效果。但冬小麦花药愈伤组织的诱导率直至目前还很低,直接影响到这一技术在小麦育种上的广泛应用。所以弄清培养基,材料(基因型)、温度等诸因素对诱导小麦花药愈伤组织的影响,是极为重要的。因此笔者对小麦花培中的培养基、材料及高温处理时间进行了研究,试图找出最佳的培养基配方和以上三处理的最优组合。 Anther culture as a new breeding method can shorten breeding generations and improve selection effect. However, the induction rate of anther callus of winter wheat is still low until now, which has a direct impact on the widespread application of this technology in wheat breeding. Therefore, it is very important to understand the effect of various factors such as medium, genotype and temperature on the induction of wheat anther callus. Therefore, the author of the wheat flower culture medium, materials and high temperature treatment time were studied in an attempt to find the best medium and the above three formulations of the optimal combination of treatment.