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AIM To explore the biological behaviors of hepatic ovacells after transfused into the circulation of experimentaanimals,METHODS:Oval cells from male SD rat were transfusedinto the circulation of a female rat which were treated by a2-AAF/CCl_4 program,through caudal vein.Sex-determininggene srywhich located on Y chromosome was examined byPCR and in situ hybridization technique in liver,kidney andspleen of the experimental animals,respectively.RESULTS:The results of the cell-transplant experimentshowed that the srygene was detectable only in the liverbut not in spleen and kidney of the experimental rats,and nosignals could be detected in the control animals,It can bealso morphologically proved that some exogenous cells hadmigrated into the parenchyma of the liver and settled there,CONCLUSION:The result means that there are exogenouscells located in the liver of the experimental animal and thelocalization is specific to the liver.This indicates that some“signal molecules”must exist in the circulation of the ratstreated by 2-AAF/CCl_4.These“signal molecules”might playan important role in specific localization and differentiationof transfused oval cells.
AIM To explore the biological behaviors of hepatic ovacells after transfused into the circulation of animal aanimals, METHODS: Oval cells from male SD rat were transfusedinto the circulation of a female rat which were treated by a2-AAF / CCl_4 program, through caudal vein. Sex- determininggene srywhich located on Y chromosome was examined by PCR and in situ hybridization technique in liver, kidney andpleen of the experimental animals, respectively .RESULTS: The results of the cell-transplant experimentshowed that the srygene was detectable only in the liverbut not in spleen and kidney of the experimental rats, and nosignals could be detected in the control animals, It can bealso morphologically proved that some exogenous cells had migrated into the parenchyma of the liver and sent there, CONCLUSION: The result means that there are exogenous cells located in the liver of the experimental animal and thelocalization is specific to the liver. This indicates that some “signal molecules ” must exist in t he circulation of the rat by at 2-AAF / CCl_4. these “signal molecules” might playan important role in specific localization and differentiation of transfused oval cells.