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目的观察脾气虚模型大鼠胃窦血管活性肠肽的含量及表达水平的变化,探讨脾主运化的现代生物学基础。方法24只SPF级SD大鼠,随机分成3组,每组8只,对照组、脾气虚模型组和四君子组,其中脾气虚组和四君子组采用饮食不节加劳倦的方法建立脾气虚模型共14天,然后通过测定体重、进食量等进行模型评价;模型建立成功后,四君子组采用四君子丸进行灌胃治疗14天,同时对照组和脾气虚模型组采用生理盐水灌胃。采用ELISA法测定血清和胃窦组织VIP含量的变化;采用RT-PCR测定胃组织VIP的mRNA表达水平变化。结果和对照组比较,脾气虚模型大鼠体重、进食量显著降低;和对照组比较,脾气虚模型组大鼠胃窦VIP含量显著升高,VIP的mRNA表达水平显著升高;和脾气虚模型组比较,四君子组大鼠胃窦VIP含量显著降低,VIP的mRNA表达水平显著降低。结论脾气虚模型大鼠胃窦VIP含量升高,VIP的mRNA表达水平显著升高可能是引起胃肠道运动功能降低,食量显著减少的机制之一。
Objective To observe the content and expression of vasoactive intestinal peptide in the gastric antrum of rats with spleen-qi deficiency model and to explore the modern biological basis of spleen main transport. Methods Twenty-four Sprague-Dawley (SD) SD rats were randomly divided into three groups (n = 8 each): control group, spleen deficiency model group and Sijunzi group. Spleen Qi deficiency group and Sijunzi group 14 days, and then through the determination of body weight, food intake and other model evaluation; model was established successfully, the Sijunzi group Sijunzi pills for gavage for 14 days, while the control group and spleen deficiency model group by saline. The contents of VIP in serum and gastric antrum were measured by ELISA. The mRNA expression of VIP in gastric tissue was detected by RT-PCR. Results Compared with the control group, the body weight and food intake of the rats with spleen-qi deficiency model were significantly decreased. Compared with the control group, the contents of VIP in the gastric antrum of the rats with spleen-qi deficiency model were significantly increased, while the mRNA expression of VIP was significantly increased. Compared with the control group, the VIP content in gastric antrum of Sijunzi group decreased significantly, and the mRNA expression of VIP decreased significantly. Conclusion The VIP content of gastric antrum in VIP rats with spleen-qi deficiency syndrome is increased, and the significant increase of VIP mRNA expression may be one of the mechanisms leading to decreased gastrointestinal motility and food intake.