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目的:建立放射性损伤的小鼠血虚证模型,观察三康胶囊的药理作用。方法:选取昆明种小鼠随机分为正常对照组、模型组、阳性对照组及三康胶囊低、中、高剂量组。采用60Co-γ射线照射的方法造成小鼠血虚证模型,分别观察给药后第14、21、28、60天外周血象以及骨髓细胞形态学改变,以此考察三康胶囊的疗效。结果:①低、中、高剂量三康胶囊给药后第14、21、28、60天对放射性损伤的小鼠血虚证所致的红细胞(RBC)、白细胞(WBC)、血小板(PLT)和血红蛋白(HB)降低均具有显著升高作用(P<0.05或P<0.01);②60天镜下观察可见:正常对照组小鼠骨髓增生极度活跃,巨核细胞、粒细胞、红细胞大小、形态正常;模型组有8例小鼠骨髓增生不良,退化细胞胞浆不完整,胞浆胞核溶解,涂片中有空泡;其余4例骨髓增生极度活跃,有大量的粒细胞(以中性杆状粒细胞、中性分叶核细胞为主),幼稚红细胞;各剂量给药组小鼠骨髓增生极度活跃,有粒细胞、晚幼粒细胞、中性杆状粒细胞、中性分叶核细胞及幼稚红细胞逐步增多;三康胶囊低、中、高剂量组分别有6、4、3例小鼠骨髓增生不良退化,细胞胞浆、胞核不完整逐步减少。阳性对照组小鼠骨髓增生极度活跃,有大量的粒细胞(以中性杆状粒细胞、中性分叶核细胞为主),幼稚红细胞;其中有3例小鼠骨髓增生不良,退化细胞胞浆、胞核不完整。结论:三康胶囊对放射性损伤的小鼠血虚证模型有一定的治疗作用。
Objective: To establish a model of blood deficiency syndrome in mice with radioactive injury and to observe the pharmacological effects of Sankang capsule. Methods: Kunming mice were randomly divided into normal control group, model group, positive control group and Sankang capsule low, medium and high dose groups. The model of blood deficiency in mice was induced by 60Co-γ ray irradiation. The peripheral blood and the morphological changes of bone marrow cells were observed on the 14th, 21st, 28th and 60th days after the administration, respectively. RESULTS: ①The RBC, WBC, PLT, blood-fat-deficient mice induced by radiation-induced blood-deficient mice on the 14th, 21st, 28th and 60th days after administration of low, medium and high- (P <0.05 or P <0.01). (2) In the observation under 60 days, the bone marrow hyperplasia in the normal control group was extremely active, the size of megakaryocyte, granulocyte, erythrocyte and morphology were normal In the model group, 8 mice had poor bone marrow hyperplasia, incomplete cytoplasm of degenerated cells, cytoplasm nucleus lysis, and vacuoles in the smear. The remaining 4 patients were extremely active in bone marrow hyperplasia with a large number of granulocytes Neutrophil granulocytes, mainly neutral), erythrocyte naïve erythrocytes. The bone marrow hyperplasia was extremely active in each dose group, including granulocyte, late promyelocytic, neutropenic granulocyte, neutral leaf core Cells and immature erythrocytes gradually increased; Sankang capsule low, medium and high dose groups were 6,4,3 mice bone marrow dysplasia degeneration, cell cytoplasm, nucleus incomplete gradually reduced. In the positive control group, myeloid hyperplasia was extremely active, with a large number of granulocytes (mainly of neutrophilic granulocytes and neutral lobular cells) and naïve erythrocytes. Three of them had poor bone marrow hyperplasia and degenerated cell Pulp, nucleus incomplete. Conclusion: Sankang Capsule has a certain therapeutic effect on blood deficiency syndrome model of mice with radioactive injury.