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为了建立新的非放射性基因标记和检测体系，用化学方法合成了Ｆｍｏｃ－苯丙氨酰氨基己酸。然后将其作为半抗原，与载体蛋白偶联（通过ＥＤＣ），用于免疫ＢＡＬＢ／ｃ小鼠。取脾细胞与Ｓｐ２／０小鼠骨髓瘤细胞融合。经ＥＬＩＳＡ筛选及有限稀释法克隆化，选出一株阳性克隆，分泌专一性抗Ｆｍｏｃ－苯丙氨酰氨基己酸的抗体。用双抗体夹心法测得其亚类为ＩｇＧ１。用间接ＥＬＩＳＡ法测得腹水效价为１．６×１０－５。用竞争性ＥＬＩＳＡ法测得亲和常数为３．６×１０６Ｍ－１。
In order to establish a new non-radioactive gene marker and detection system, Fmoc-phenylalanyl aminocaproic acid was chemically synthesized. This was then used as a hapten, coupled to the carrier protein (via EDC) for immunization of BALB / c mice. Spleen cells were spliced to Sp2 / 0 mouse myeloma cells. After screening by ELISA and limiting dilution, a positive clone was selected to secrete specific anti-Fmoc-phenylalanyl amino caproic acid antibody. Its subclass was IgG1 measured by double antibody sandwich method. Ascites titer measured by indirect ELISA was 1.6 × 10-5. The affinity constant was 3.6 × 10 6 M -1 by competitive ELISA.