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目的采用HPLC法测定排毒胶囊中大黄素和大黄素甲醚的含量。方法采用Wondisol C18色谱柱(250 mm×4.6mm,5μm),柱温35℃,流动相为甲醇-0.1%磷酸溶液,以1.0 m L·min-1流速梯度洗脱,检测波长254 nm。结果大黄素和大黄素甲醚的线性范围分别为7.0~105.0、2.0~30.0μg·m L-1(r分别为0.9999、0.9992);加样回收率分别为102.2%、99.2%(n=6)。结论所用方法操作简单、结果准确、重复性好,可有效地控制该制剂的内在质量。
Objective To determine the content of emodin and emodin in Detox capsule by HPLC. Methods A Wondisol C18 column (250 mm × 4.6 mm, 5 μm) was used. The column temperature was 35 ℃. The mobile phase consisted of methanol - 0.1% phosphoric acid solution and eluted with a gradient of 1.0 mL · min -1. The detection wavelength was 254 nm. Results The linear ranges of emodin and physcion were 7.0 ~ 105.0 and 2.0 ~ 30.0 μg · m L-1 (r = 0.9999 and 0.9999, respectively). The recoveries were 102.2% and 99.2% (n = 6 ). Conclusion The method used is simple, accurate and reproducible. It can effectively control the internal quality of the preparation.