论文部分内容阅读
AIM:To explore the role of hepatitis C virus(HCV)envelopeprotein 2(E2)in the induction of apoptosis.METHODS:A carboxyterminal truncated E2(E2-661)wastransiently expressed in several cultured mammalian celllines or stably expressed in Chinese hamster ovary(CHO)cell line.Cell proliferation was assessed by ~3H thymidineuptake.Apoptosis was examined by Hoechst 33258staining,flow cytometry and DNA fragmentation analysis.RESULTS:Reduced proliferation was readily observed inthe E2-661 expressing cells.These cells manifested thetypical features of apoptosis,including cell shrinkage,chromatin condensation and hypodiploid genomic DNAcontent.Similar apoptotic cell death was observed in anE2-661 stably expressing cell line.CONCLUSION:HCV E2 can induce apoptosis in culturedmammalian cells.
AIM: To explore the role of hepatitis C virus (HCV) envelope protein 2 (E2) in the induction of apoptosis. METHODS: A carboxyterminal truncated E2 (E2-661) wastransiently expressed in several cultured mammalian cell lines or stably expressed in Chinese hamster ovary ( CHO) cell line. Cell proliferation was assessed by ~ 3H thymidine uptake. Apoptosis was examined by Hoechst 33258 staining, flow cytometry and DNA fragmentation analysis. RESULTS: Reduced proliferation was readily observed in E2-661 expressing cells. The se cells manifested thetypical features of apoptosis, including cell shrinkage, chromatin condensation and hypodiploid genomic DNA content. similar apoptotic cell death was observed in an E2-661 stably expressing cell line. CONCLUSION: HCV E2 can induce apoptosis in cultured mammalian cells.