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醋酸纤维薄膜电泳测定血红蛋白A_2(HbA_2)方法简便,分离快速,洗脱完全,便于推广。本文介绍醋酸纤维薄膜电泳测定方法,以及正常人和各种血液病患者 HbA_2的测定结果。对象、材料和方法一、对象:正常人42例系本院的健康工作人员和供血员。血液病患者69例均经临床和血液学检查确诊(详见表1)。测定时采血1—3毫升,置刻度试管中以枸椽酸盐抗凝,离心,去血浆后用生理盐水将红细胞洗涤三次,然后加入与红细胞压积等体积的蒸馏水
Acetate film electrophoresis determination of hemoglobin A_2 (HbA_2) method is simple, fast separation, complete elution, easy to promote. This article describes the determination of cellulose acetate membrane electrophoresis, as well as normal and various blood disease patients HbA_2 determination results. Objects, materials and methods First, the object: 42 cases of normal people in our hospital health workers and blood donors. 69 cases of hematological diseases were diagnosed by clinical and hematological examination (see Table 1). Determination of blood 1-3 ml, set the scale to anticoagulant citrate in the test tube, centrifuged, after the plasma to wash the erythrocytes with saline three times, and then added with the hematocrit equal volume of distilled water