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为了探究腐植酸(HA)对褐鳟鱼鳃和肌肉在抵抗镉(Cd)中毒方面是否发挥积极作用,进行了组织病理学和生物化学方面的研究。试验中,褐鳟暴露在仅施加2 ppm镉,施加2 ppm镉加5 ppm腐植酸,仅施加5 ppm腐植酸的3种环境条件下,分别测定其鳃和肌肉组织的谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平。在含镉条件下,褐鳟组织中的GPx和SOD活性显著低于对照组(P<0.05),而含镉环境中褐鳟组织的MDA并未升高(P>0.05)。然而,腐植酸并未影响镉造成的生化伤害。镉对鱼鳃和肌肉组织造成明显的组织病理学变化,而在镉+腐植酸处理组中,肌肉组织病理学变化较小。这些结果表明,腐植酸可从组织病理学方面中和肌肉组织镉中毒。
To investigate whether humic acid (HA) plays a positive role in the gill and muscle of brown trout against cadmium (Cd) poisoning, histopathological and biochemical studies were performed. In the experiment, brown trout was exposed to glutathione peroxidation of gill and muscle tissue under 3 environmental conditions of applying only 2 ppm of cadmium, 2 ppm of cadmium plus 5 ppm of humic acid, and 5 ppm of humic acid alone (GPx), superoxide dismutase (SOD) and malondialdehyde (MDA). The activities of GPx and SOD in brown trout tissues were significantly lower than those in the control (P <0.05), while MDA in brown trout tissues was not increased in the cadmium containing environments (P> 0.05). However, humic acid did not affect the biochemical damage caused by cadmium. Cadmium caused significant histopathological changes in fish gills and muscular tissues, while in the cadmium + humic acid treatment groups, muscle tissue pathological changes were small. These results indicate that humic acid neutralizes muscular tissue cadmium poisoning from histopathology.