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[目的]了解我国沿海野生菲律宾蛤仔群体遗传多样性和遗传分化情况。[方法]采用PCR技术对烟台威海2个群体的线粒体COⅠ基因片段进行扩增和序列分析。[结果]50个样本均得到684bp的序列,共定义了42个单倍型,检测到62个变异位点,2个群体单倍型多样性都较高,而核苷酸多样性相对偏低。用最大似然法(Maximum Likelihood,ML)构建的单倍型系统进化树显示2个地理种群的样本呈现散在分布,没有明显的种群分化。[结论]反映了海洋动物的基因交流较容易,不同海域隔离较弱。
[Objective] To understand the genetic diversity and genetic differentiation of wild clams in the Philippines. [Method] The mitochondrial COI gene fragment of two populations in Weihai of Yantai was amplified by PCR and sequenced. [Result] The sequences of 684bp were obtained in all 50 samples. A total of 42 haplotypes were defined and 62 loci were detected. The haplotype diversity of the two populations were high and the nucleotide diversity was relatively low . The phylogenetic tree of haplotypes constructed by Maximum Likelihood (ML) showed that the samples of two geographic populations showed scattered distribution with no obvious population differentiation. [Conclusion] It reflects that the gene exchange of marine animals is easy and the isolation in different sea areas is weak.