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[目的]建立湖南省健康儿童各年龄阶段外周血淋巴细胞免疫表型的正常参考范围.[方法]选取2011年3月至2011年12月在本院儿童保健所健康体检的儿童667例,年龄1个月至15岁,其中男397名,女270名.将健康体检儿童分为1~6个月、6~12个月、1~2岁、2~3岁、3~15岁五个年龄组,采用流式细胞技术检测各年龄组免疫表型 CD3+、CD4+、CD8+、CD19+、CD16+56+百分率.[结果] ① 1~6个月年龄组 CD3+ T 细胞、CD4+ T 细胞和 CD16+56+ NK 细胞与 6~12个月、1~2岁、2~3岁、3~15岁年龄组间比较有显著统计学意义( P <0.01),1至6月年龄组 CD3+ T 细胞、CD4+ T 细胞值高于其他年龄组,CD16+56+ NK 淋巴细胞低于其他年龄组.② CD4+ T 细胞及 CD4+ /CD8+比值随年龄增长有下降趋势,CD16+56+ NK细胞有升高趋势,其余淋巴细胞免疫表型在6月龄后相对保持稳定 . ③ 6~12个月、1~2岁和2~3岁三个年龄组间比较无明显规律性趋势,无统计学意义占多数,将三组合并为6个月至3岁一个年龄组 . 1~6个月、6个月至3岁及 3~15岁三组之间除了 1~6个月与 6个月至3岁 、3~15岁年龄组 CD19+ B 细胞和 6个月至3岁与 3~15岁年龄组的 CD3+ T 细胞无统计学意义外,其他各年龄组免疫表型比较均有统计学意义( P <0.05).[结论]儿童淋巴细胞免疫表型的百分率与年龄相关,本研究以 1~6个月、6个月至3岁及 3~15岁三个年龄组建立健康儿童淋巴细胞免疫表型正常参考范围为临床提供参考.“,”Objective] To establish the normal reference range of lymphocyte immunophenotypes in peripheral blood of different a ‐ges of healthy children in Hunan province .[Methods] Totally 667 children(397 boys and 270 girls) aged one month to 15 years receiv‐ing health examination in our hospital from March 2011 to Dec .2011 were enrolled in the study .Healthy children were divided into five age groups including 1 ~ 6 months group ,6 ~ 12 months group ,1 ~ 2 years old group ,2 ~ 3 years old group and 3 ~ 15 years old group .The percentage of CD3 + ,CD4 + ,CD8 + ,CD19 + and CD16 + CD56 + lymphocytes in peripheral blood of each age group were de‐tected by flow cytometry .[Results] There were significant differences in the percentage of CD 3 + and CD4 + T‐lymphocytes and CD16 +CD56 + NK cells between 1 ~ 6 months group and 6 ~ 12 months group ,1 ~ 2 years old group ,2 ~ 3 years old group and 3 ~ 15 years old group( P < 0 .01) .The percentage of CD3 + and CD4 + T‐lymphocytes in 1 ~ 6 months group were higher than those in other groups ,while the percentage of CD16 + CD56 + NK cells were lower than those in other groups .The percentage of CD4 + lymphocytes and the ratio of CD4 + /CD8 + decreased with the age ,but the percentage of CD16 + CD56 + lymphocytes increased with age .After 6 months old ,the percentage of other lymphocytes was relatively stable .There was no obvious regular trend among 6 ~ 12 month group , 1 ~ 2 years old group and 2 ~ 3 years old group ,and the difference had no statistical significance .Above three groups were combined in‐to one group(6 months to 3 years old group) .Except there was no significant difference in CD 19 + B‐lymphocytes among 1 ~ 6 months group ,6 months to 3 years old group and 3 ~ 15 years old group and CD3 + T‐lymphocytes between 6 months to 3 years old group and 3 ~ 15 years old group ,there were significant differences in immunophenotypes among other age groups ( P < 0 .05) .[Conclusion]The percentage of lymphocyte immunophenotypes in children are associated with age .The study suggests that the normal reference ranges of lymphocyte immunophenotypes for healthy children according 1 ~ 6 months ,6 months to 3 years old and 3 ~ 15 years old should be es‐tablished to provide for clinical reference .