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在清醒自由活动的SD雄性大鼠中,第三脑室内微量注射胃泌素释放肽(GRP)0.7和2.0μg后,兴奋前叶垂体β-内啡肽(β-EP)的静启分泌;2.0μg GRP可进一步兴奋限制性应激引起的β-EP释放。上述两个剂量的GRP都抑制催乳素(PRL)的静息分泌和应激时PRL的释放。 在离体培养的前叶垂体细胞中,0.5和1.0μg的GRP都兴奋B-EP和PRL的分泌。结果提示,GRP抑制PRL释放的作用地点在下丘脑,而GRP兴奋β-EP释放的作用地点可能在下丘脑或直接作用于垂体。
In awake free-moving SD male rats, 0.7 and 2.0 μg of gastrin-releasing peptide (GRP) were injected into the third ventricle to excite the secretory secretion of β-EP in the anterior pituitary. 2.0 μg GRP further excites β-EP release caused by restrictive stress. Both doses of GRP inhibited the resting secretion of prolactin (PRL) and the release of PRL at stress. In ex vivo cultured anterior pituitary cells, both 0.5 and 1.0 μg of GRP were excited by the secretion of B-EP and PRL. The results suggest that the role of GRP in inhibiting the release of PRL in the hypothalamus, while the role of GRP excited β-EP release may be in the hypothalamus or directly on the pituitary gland.