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目的:探讨2009年新型甲型H1N1流感病毒神经氨酸酶(NA)基因的进化及NA基因编码蛋白抗原性、酶活性位点、糖基化位点变异情况。方法:从NCBI基因库检索获得43株不同年代不同地域甲型流感病毒NA基因序列,用Molecular Evolutionary Genetics Analysis version 4.0(MEGA4.0)软件进行基因进化分析和氨基酸序列分析。结果:2009年新型甲型H1N1流感病毒与禽H5N1流感病毒NA基因的同源性达到85%,潜在抗原位点氨基酸分布相同;所有毒株的酶活性中心位点高度保守,但糖基化位点有变异。结论:2009年新型甲型H1N1流感病毒的NA基因可能来源于禽H5N1流感病毒;神经氨酸酶抑制剂治疗有效。
Objective: To investigate the evolution of neuraminidase (NA) gene of new type A H1N1 influenza virus in 2009 and the variation of antigenicity, enzyme active site and glycosylation site of NA protein. Methods: NA gene sequences of 43 strains of influenza A virus from different regions of China were obtained from the NCBI gene library. The molecular evolutionary analysis and amino acid sequence analysis were performed with Molecular Evolutionary Genetics Analysis version 4.0 (MEGA4.0) software. Results: The homology of the NA gene between the new type A H1N1 influenza virus and the avian H5N1 influenza virus was 85% in 2009, and the amino acid distributions of the potential antigenic sites were the same. The enzyme activity was highly conserved among all the strains, but the glycosylation sites There are variations. Conclusion: The NA gene of the new type A H1N1 influenza virus in 2009 may be derived from the avian H5N1 influenza virus; neuraminidase inhibitors are effective.