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本实验利用蛞蝓干粉浸出液,处理体外培养A_(540)肺癌细胞,通过连续四次细胞计数,制成肺癌细胞生长曲线,表示蛞蝓对肺癌细胞生长率的影响。实验结果证明,培养液中药物的最终浓度在0.6%时,培养六天,肺癌细胞数目显著减少。与对照组比较,P<0.05,差异有显著性意义。不仅如此,实验组细胞形态结构亦有所变化。说明蛞蝓浸出液在一定浓度时,对肺癌细胞具有明显的抑制作用。
In this experiment, A_(540) lung cancer cells were cultured in vitro using maggot dry extract and the growth curve of lung cancer cells was determined by four consecutive cell counts, indicating the effect of earthworms on the growth rate of lung cancer cells. The experimental results show that when the final concentration of the drug in the culture medium is 0.6%, the number of lung cancer cells is significantly reduced after 6 days of culture. Compared with the control group, P<0.05, the difference was significant. Not only that, the morphology and structure of the cells in the experimental group also changed. It shows that the leaching solution has a significant inhibitory effect on lung cancer cells at a certain concentration.