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[目的]筛选出防治番石榴实蝇Beauveria bassiana(Bezzi)的有效生防菌。[方法]分别采用喷雾法和浸渍法测定不同浓度的球孢白僵菌Bb080920分生孢子液对番石榴实蝇成虫、幼虫和蛹的室内毒力。[结果]成虫的最大校正死亡率达(91.83±2.42)%,致死中浓度(LC50值)为(2.6281±0.2362)×108个/L,致死中时间(LT50值)为(4.61±0.37)d;幼虫最大累计校正死亡率达(95.23±3.13)%,LC50值为(2.5416±0.8395)×108个/L,LT50值为(4.36±0.42)d;蛹的累计校正死亡率最大达(82.59±2.78)%,LC50值为(2.7692±0.1538)×108个/L,LT50值为(5.05±0.68)d。[结论]球孢白僵菌Bb080920菌株毒力测定表明具有很高的毒性,未来有良好的应用前景。
[Objective] The research aimed to screen out the effective biocontrol agents against Beauveria bassiana (Bezzi). [Method] The indoor toxicity of conidia of Beauveria bassiana Bb080920 with different concentrations to adults, larvae and pupae of fruit fly guadalajes was determined by spray method and immersion method respectively. [Result] The maximum corrected mortality of adults was (91.83 ± 2.42)%, the lethal medium concentration (LC50) was (2.6281 ± 0.2362) × 108 cells / L and the lethal time was (4.61 ± 0.37) d (95.23 ± 3.13)%, the LC50 value was (2.5416 ± 0.8395) × 108 cells / L and the LT50 value was (4.36 ± 0.42) d. The cumulative corrected mortality of pupae was the highest (82.59 ± 2.78%), the LC50 value was (2.7692 ± 0.1538) × 108 cells / L and the LT50 value was (5.05 ± 0.68) d. [Conclusion] The virulence of Bb080920 strain of Beauveria bassiana showed high toxicity and good prospect in the future.