在确定二价铁离子为A.f生长过程中惟一限制性底物条件下,通过考察初始亚铁离子浓度、初始pH值两种影响亚铁离子氧化代谢的主要因素来研究细菌的生长特性,得到以限制性底物亚铁离子浓度为表征的细菌生长曲线。利用基于Monod方程建立的细菌生长动力学方程模型,采用Matlab软件中的Gauss-Newton算法确定了在不同条件下细菌生长动力学参数,包括最大比生长速率μm、Monod常数K及Ro,推导出了不同条件下A.f对数期以底物Fe(Ⅱ)浓度为表征的生长动力学方程。 In the determination of ferrous iron as the only limiting substrate in the growth of Af, the growth characteristics of the bacteria were studied by examining the primary ferrous ion concentration and initial pH, two of the main factors affecting the ferrous ion oxidation metabolism. The limiting substrate ferrous ion concentration is the characterization of the bacterial growth curve. Based on the model of bacterial growth kinetics established by Monod equation, the parameters of bacterial growth kinetics under different conditions, including the maximum specific growth rate μm, Monod constants K and Ro, were determined by Gauss-Newton algorithm in Matlab software Under different conditions, Af logarithmic phase is characterized by growth of substrate Fe (Ⅱ).