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目的探讨过敏性紫癜(HSP)患儿血淋巴细胞亚群变化的意义。方法采用双标法在流式细胞仪上检测61例住院HSP患儿血淋巴细胞亚群CD3+、CD3+CD4+、CD3+CD8+、CD4+/CD8+、CD3-CD19+、CD3-(CD16+56+)、CD19+CD23+水平,并与同期19例同龄健康儿童作对照;采用速率法应用HIT蛋白自动分析仪测定其中53例HSP患儿血清IgA及其淋巴细胞亚群;采用速率法、应用OLYMPUSAU400自动分析仪测定其中45例HSP患儿尿微量清蛋白、尿肌酐、N-乙酰-β-氨基-葡萄糖苷酶(NAG)。应用SAS6.12软件进行统计学分析。结果HSP组与健康对照组比较血CD3+CD4+、CD4+/CD8+、CD3-(CD16+56+)明显降低(Pa<0.05),CD3-CD19+、CD19+CD23+明显升高,CD3+、CD3+CD8+无显著性差异。血清IgA水平与淋巴细胞亚群无相关性(r=0.224P>0.05),以尿微量清蛋白/尿肌酐(UmAlb/Cr)(mg/g)=30为界限,NAG以11.9U/L为界限,分别将淋巴细胞亚群分二组,结果显示UmAlb/Cr>30与≤30组比较,NAG>11.9与≤11.9组比较,CD19+CD23+均明显升高[(6.71±0.80)vs(5.78±0.46),(9.36±1.14)vs(5.44±0.50)Pa<0.05]。结论HSP急性期存在免疫功能紊乱,尤其是活化的B淋巴细胞(CD19+CD23+)明显升高,并且其可能与肾损伤程度有一定关系。
Objective To investigate the significance of changes of lymphocyte subsets in children with Henoch-Schonlein purpura (HSP). Methods The CD3 +, CD3 + CD4 +, CD3 + CD8 +, CD4 + / CD8 +, CD3 + CD19 +, CD3- (CD16 + 56 +) were detected by flow cytometry in 61 children with HSP in hospital. CD19 + CD23 +, and compared with 19 healthy children of the same age in the same period. The serum IgA and lymphocyte subsets of 53 children with HSP were measured by HIT protein autoassay using rate method. Using the speed method, the OLYMPUSAU400 automatic analyzer Urine microalbuminuria, urinary creatinine and N-acetyl-β-amino-glucosidase (NAG) were measured in 45 children with HSP. SAS6.12 software was used for statistical analysis. Results The levels of CD3 + CD4 +, CD4 + / CD8 + and CD3- (CD16 + 56 +) in HSP group were significantly lower than those in healthy control group (Pa0.05), CD3-CD19 + and CD19 + CD23 + Significant difference. There was no correlation between serum IgA level and lymphocyte subsets (r = 0.224P> 0.05), UmAlb / Cr (mg / g) = 30, NAG was 11.9U / L Lymphocyte subsets were divided into two groups, the results showed that UmAlb / Cr> 30 and ≤30groups, NAG> 11.9and≤11.9groups, CD19 + CD23 + were significantly higher (6.71 ± 0.80vs vs5.78 ± 0.46), (9.36 ± 1.14) vs (5.44 ± 0.50) Pa <0.05]. Conclusions Immune dysfunction exists in the acute phase of HSP, especially the activated B lymphocyte (CD19 + CD23 +) is obviously increased, and it may be related to the degree of renal injury.