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目的为提高肝癌患者生存期和改善生活质量,探索研究纳米磁诱导肝癌Hep-6细胞凋亡,提供肝局部消融新方法。方法应用电泳,扫描电镜,透射电镜及流式细胞仪技术。实验设计分组为空白对照组;药物对照组;纳米磁处理组;在不同药物剂量与作用时间条件下重点观察纳米磁诱导人肝癌Hep-6细胞凋亡改变。结果①药物剂量:纳米磁组与表阿霉素对照组,再分为高剂量与低剂量组,按10mg/L,100mg/L终浓度作用人肝癌Hep-6细胞。②24h的结果实验组细胞凋亡率由25%上升到54%;36h的结果实验组和表阿霉素组与对照组的凋亡率分别为78%,53%,2%;各组结果之间的凋亡率差异显著(t=3.05,P<0.05),凋亡率和药量与时间呈正相关联(r=0.96,P<0.05)。结论纳米磁具有高载药量、靶向性、缓释作用,可诱导肝癌Hep-6细胞凋亡。
Objective To improve the survival of patients with liver cancer and improve the quality of life, to explore the nano-magnetic induction of Hep-6 hepatoma cell apoptosis, to provide a new method of local liver ablation. Methods Electrophoresis, scanning electron microscopy, transmission electron microscopy and flow cytometry were used. The experimental design was divided into blank control group, drug control group and nano-magnetic treatment group. The apoptosis of Hep-6 cells induced by nano-magnetism was observed under different dose and duration of action. Results ① The drug dose: nano-magnetic group and epirubicin control group, divided into high-dose and low-dose group, according to 10mg / L, 100mg / L final concentration of human hepatoma Hep-6 cells. ②24h results in the experimental group apoptosis rate increased from 25% to 54%; 36h results in the experimental group and the epirubicin group and the control group apoptosis rates were 78%, 53%, 2%; Results of each group There was a significant difference between the two groups (t = 3.05, P <0.05). There was a positive correlation between apoptosis rate and dose and time (r = 0.96, P <0.05). Conclusion Magnetic nanoparticles with high drug loading, targeting, sustained release, can induce apoptosis of Hep-6 cells.