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为有效延缓东亚飞蝗对马拉硫磷的抗药性,明确相关解毒代谢酶在抗药性产生过程中的动态变化,本试验在马拉硫磷对东亚飞蝗3龄蝗蝻毒力测定的基础上,测定了相关解毒代谢酶的活力。结果表明,马拉硫磷处理48h后其LC50值为448.89mg/L。马拉硫磷低浓度(7.48-187mg/L)显著抑制东亚飞蝗乙酰胆碱酯酶、全酯酶和CDNB为底物时谷胱甘肽-S-转移酶的活性,处理间酶活性随着马拉硫磷浓度的升高出现先降低后升高的趋势;马拉硫磷对DCNB为底物时谷胱甘肽-S-转移酶活性呈显著诱导作用,处理间酶活随马拉硫磷浓度升高出现先升高后降低的趋势;马拉硫磷对多功能氧化酶活性影响差异不显著。以上结果说明,乙酰胆碱酯酶、全酯酶和谷胱甘肽-S-转移酶均参与了东亚飞蝗对马拉硫磷的解毒代谢过程;马拉硫磷通过抑制昆虫体内相关解毒代谢酶活性从而达到其杀虫效果。综合前人对马拉硫磷抗性研究结果可以看出,以上生化酶抑制剂的添加可以在一定程度上延缓昆虫对马拉硫磷抗性的产生,并在抗性治理中发挥一定作用。
In order to effectively delay the resistance of Locusta migratoria manilensis to malathion and to clarify the dynamic changes of related detoxification metabolic enzymes in the process of drug resistance, based on the determination of 3-year-old locust virulence of malathion to locust , The activity of the relevant detoxifying metabolic enzymes was determined. The results showed that the LC50 value of malathion after 48h was 448.89mg / L. The low concentration of malathion (7.48-187 mg / L) significantly inhibited the activity of acetylcholinesterase, total esterase and CDNB as substrates in E.coli, The increase of lathion concentration firstly decreased and then increased, while malathion significantly induced glutathione-S-transferase activity when DCNB was substrate. The concentration increased first and then decreased. Malathion had no significant effect on the activity of multifunctional oxidase. The above results show that acetylcholinesterase, all esterase and glutathione-S-transferase are involved in the detoxification process of malathion in Locusta migratoria manilensis; malathion inhibits the activity of detoxification and metabolic enzymes in insects To achieve its insecticidal effect. Based on the results of previous studies on malathion resistance, it can be seen that the addition of the above biochemical enzyme inhibitors can delay the production of malathion to insects to a certain extent and play a role in resistance management.