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以每日5mg/kg的3-氯丙二醇及75mg/kg的2,3-氧丙醇同时给大鼠灌胃,连续给药2天后停药8天,共10天为一疗程。观察了3,6,9疗程结束时,附睾起始部主细胞的超微结构改变,及附睾管起始部的精子和肝脏、肾脏的超微结构。发现在3、6疗程末,附睾主细胞的高尔基复合体扁平囊变窄,大泡皱缩。在6疗程末,细胞表面的吞饮小泡和静纤毛减少。仅在3疗程见到粗面内质网扩张。9疗程的超微结构与对照比较无明显区别。结果提示,每一疗程药物引起的附睾主细胞超微结构的改变,在多数动物是可恢复的。但由于个体差异,部分动物于疗程之末,尚未完全恢复。在第6疗程末的肝脏,有部分肝细胞滑面内质网扩张,线粒体体积缩小,基质电子密度增高。这些变化可能是一种轻度的细胞损伤,这种损伤在3,9疗程均未发现,说明它与疗程长短不呈平行关系。肾尿细管上皮细胞及附睾起始部精子的超微结构,无可见改变。这一结果为阐明3-氯丙二醇及2,3-氧丙醇合并用药的抗生育作用,及对有关内脏的毒性作用,提供了形态学的依据。
The rats were intragastrically administered with 5-chloropropanediol (5 mg/kg) and 2,3-oxopropanol (75 mg/kg) at the same time. The drug was discontinued for 8 days after continuous administration for 2 days for 10 days as a course of treatment. At the end of the 3,6,9 treatment period, the ultrastructural changes of the main cells in the epididymal origin and the ultrastructure of the sperm and liver and kidney in the epididymal tube were observed. At the end of the 3rd and 6th courses, it was found that the Golgi complex’s flat sac of the epididymal main cell narrowed and the large bubble shrank. At the end of the 6th cycle, there were fewer blebs and cilia on the cell surface. The rough endoplasmic reticulum was seen only in 3 courses. There was no significant difference between the ultra-structure of 9 courses and the control. The results suggest that the ultrastructural changes in the epididymal main cells caused by each course of treatment are recoverable in most animals. However, due to individual differences, some animals have not fully recovered at the end of their treatment. In the liver at the end of the 6th treatment period, part of the hepatocyte slipped endoplasmic reticulum, the mitochondrial volume decreased, and the matrix electron density increased. These changes may be a mild form of cell damage that was not detected during the 3 and 9 treatments, suggesting that it is not parallel to the length of treatment. The ultrastructure of the renal urinary tubule epithelial cells and the spermatozoa in the epididymis initiating part showed no visible changes. This result provides a morphological basis for the elucidation of the antifertility effects of the combined use of 3-chloropropanediol and 2,3-oxopropanol and its toxic effects on visceral organs.