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本文报告了以SPA亲和层析、pH梯度洗脱技术纯化McAb的效果,纯化物以SDS—聚丙烯酰胺电泳、ELISA及中和试验检测。也报告了以免疫双扩散沉淀技术对脊髓灰质炎(polio)Sabin—1,IS—11株McAb之IgG亚类,轻链型别鉴定的结果。
In this paper, we report the purification of McAb by SPA affinity chromatography and pH gradient elution. The purified compounds were detected by SDS-polyacrylamide electrophoresis, ELISA and neutralization assay. The results of immunodominant double-diffusion precipitation on the IgG subclass and light chain type of polio Sabin-1 and IS-11 McAbs were also reported.