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目的建立香港海鸥形菌(Laribacer hongkongensis)的基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)鉴定方法,并对其实验室应用加以评价。方法用VITEK MS全自动快速微生物质谱检测系统对12株香港海鸥形菌进行鉴定和分析,用SARAMIS数据库构建参考图谱;并用新分离的香港海鸥形菌、嗜水气单胞菌、温和气单胞菌、溶血不动杆菌、琼氏不动杆菌、腐败希瓦氏菌、藤黄微球菌、类志贺邻单胞菌等表型相似的菌株对所建立的鉴定方法进行验证。结果本实验构建的香港海鸥形菌参考图谱特异性高、重复性好,是细菌鉴定数据库中未见的新图谱;新分离香港海鸥形菌菌株均能被MS成功鉴定,且图谱吻合率在98.5%以上,其他25株表型相似菌株均能被成功鉴别。结论MALDI-TOF-MS技术能快速和准确地鉴定香港海鸥形菌,此方法可用于香港海鸥形菌的临床快速诊断或食品安全性评价。
Objective To establish a method for the identification of Laribacer hongkongensis by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) and evaluate its application in laboratory. Methods 12 strains of Hong Kong gull were identified and analyzed by VITEK MS automated rapid microbial mass spectrometry system. SARAMIS database was used to construct the reference map. The new isolated H. guppy, Aeromonas hydrophila, Bacteroides, Acinetobacter baumannii, Acinetobacter jaundice, Shewanella spp., Micrococcus luteus, Pseudomonas schizophyllum and other similar phenotypes were used to verify the identification method. Results The reference map of H. guppy was constructed with high specificity and repeatability in this study. It was a new profile not found in the bacterial identification database. The newly isolated H. guppy could be successfully identified by MS with the coincidence rate of 98.5 % Of the other 25 strains of similar phenotype can be successfully identified. Conclusion The MALDI-TOF-MS technique can rapidly and accurately identify H. guppy in Hong Kong. This method can be used for clinical rapid diagnosis or food safety evaluation of H. guppy in Hong Kong.