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本文采用生物毒力和酶活力测定法,研究了棉红铃虫(Pectino phora gossypiella Saunders)雌雄幼虫的耐药性差异及其代谢机制。结果表明,安庆种群的耐药性具有明显的性别差异,但差异程度与药剂种类有关,雌性幼虫对有机磷剂和拟菊酯类的50%死亡率(LD50)值分别是雄性个体的1.7～5.7倍和1.8～28.2倍.增效剂(Pb 和 TPP)对溴氰菊酯均有很强的增效作用,但对雌性幼虫的增效作用显著高于雄性。雌性幼虫的多功能氧化酶(MFO)、酯酶(ES7)和谷胱甘肽转移酶(GST)的活力及谷胱甘肽(GSH)含量分别为雄性幼虫的2.41、1.67、1.12和2.72倍,说明棉红铃虫的耐药性具有性别差异,且解毒代谢是其差异的主要原因。
In this study, the bioassay and enzyme activity assay were used to investigate the differences in drug resistance and the metabolic mechanisms of the female and male larvae of Pectino phora gossypiella Saunders. The results showed that there was a significant gender difference in the drug resistance of Anqing population, but the degree of difference was related to the type of medicament. The 50% mortality rate (LD50) of the organophosphorus pesticide and the herbicide of the pyrethroids in female larvae was 1.7 ~ 5.7 times and 1.8 ~ 28.2 times.The synergist (Pb and TPP) have a synergistic effect on deltamethrin, but synergistic effect on female larvae was significantly higher than male. The activities and glutathione (GSH) contents of MFO, ES7 and GST of female larvae were 2.41, 1.67, 1.12 and 2.72 times higher than that of male larvae respectively , Indicating that the resistance of cotton bollworm has gender differences, and detoxification metabolism is the main reason for the difference.