论文部分内容阅读
采用Kirby-Bauer纸片扩散法和随机扩增多态性DNA(Random Amplified Polymorphic,RAPD)分型法对23株水源性铜绿假单胞菌进行耐药性及遗传多样性研究。药敏实验结果显示,23株分离株对磺胺甲基异恶唑/甲氧苄氨嘧啶(SXT)、四环素(TE)、米诺环素(MH)的耐药率分别为69.4%、13.2%和39.2%,对另外13种抗生素的敏感性几乎100%。RAPD-PCR指纹图谱聚类分析显示,在相对系数为62%时,引物208将24株菌分为5簇(A~E),其中C为主要的簇,引物272将24株菌分为4簇(F~I),其中I和G簇为主要的簇。23株分离株中无耐药菌株主要集中在B簇,3株多重耐药菌株集中在D簇,对SXT、MH耐药菌株主要集中在I簇。本研究发现水源性铜绿假单胞菌具有较高的遗传多样性,且存在多重耐药,为水源性铜绿假单胞菌的污染溯源和控制提供了相应的数据支持。
The drug resistance and genetic diversity of 23 strains of Pseudomonas aeruginosa were studied by Kirby-Bauer disk diffusion method and Random Amplified Polymorphic (RAPD) typing. The results of drug sensitivity test showed that the resistance rates of 23 isolates to SXT, TE and MH were 69.4% and 13.2% respectively, And 39.2% respectively, almost 100% sensitivity to the other 13 antibiotics. RAPD-PCR fingerprinting cluster analysis showed that at the relative coefficient of 62%, primer 208 divided 24 strains into 5 clusters (A ~ E), among which C was the main cluster, and primer 272 divided 24 strains into 4 Clusters (F ~ I), where I and G clusters are the major clusters. Among the 23 isolates, no drug-resistant strains were concentrated in cluster B, three multi-drug resistant strains were clustered in cluster D, while those susceptible to SXT and MH were mainly clustered in cluster I. This study found that water-soluble Pseudomonas aeruginosa has a high genetic diversity, and there is multi-drug resistance, which provides data support for the traceability and control of water-source Pseudomonas aeruginosa.