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为探讨褪黑素(MT)对哮喘小鼠转化生长因子β(TGF-β)的影响及其在气道重构中的作用,将小鼠40只随机分成正常对照组、哮喘模型组、褪黑素治疗组(MT组)、地塞米松治疗组(DXM组),每组10只。哮喘组、MT组和DXM组用卵蛋白(OVA)致敏小鼠并反复雾化吸入OVA达4周,MT组和DXM组小鼠分别给予腹腔注射MT、DXM。各组于最后1次雾化激发后进行取材,收集肺组织,制作石蜡切片,HE染色观察气道中嗜酸粒细胞;Masson三色染色法观察气道周围胶原沉积情况;PAS染色法观察气道黏液分泌情况;用Leica软件测定单位气道面积基底膜周径(Pbm)、管壁总面积(WAt)、内壁面积(WAi)、平滑肌面积(WAm)、胶原面积(Wcol)及黏液分泌面积;用RT-PCR方法检测肺组织中TGF-βmRNA水平。结果表明,哮喘模型组、MT组和DXM组WAt/Pbm、WAi/Pbm、WAm/Pbm、Wcol/Pbm和黏液分泌面积均高于正常对照组,哮喘组亦高于MT组和DXM组(P值均<0.05),MT组与DXM组间无显著差异(P>0.05)。哮喘组、MT组和DXM组TGF-βmRNA均高于正常对照组,哮喘组亦高于MT组和DXM组(P均<0.01),MT组与DXM组间无显著差异(P>0.05)。以上表明,MT能降低哮喘小鼠肺组织TGF-βmRNA水平,部分抑制气道重构,其调节作用与地塞米松相当。
To investigate the effect of melatonin (MT) on transforming growth factor-β (TGF-β) and its role in airway remodeling in asthmatic mice, 40 mice were randomly divided into normal control group, asthma model group, Melanin treatment group (MT group), dexamethasone treatment group (DXM group), each group of 10. The asthmatic group, MT group and DXM group were sensitized with ovalbumin (OVA) and repeatedly inhaled OVA for 4 weeks. The MT and DXM groups were given intraperitoneal injection of MT and DXM respectively. The rats in each group were harvested after the last atomization and the lung tissues were collected to make paraffin sections. The eosinophils in the airways were observed by HE staining, the collagen deposition in the airways was observed by Masson’s trichrome staining, the airway was observed by PAS staining The mucosal secretion was measured by using Leica software. The perimeter of basement membrane was measured by Leica software. The peritumoral diameter (Pbm), total wall area (WAt), inner wall area (WAi), smooth muscle area (WAm), mucus secretion area The level of TGF-βmRNA in lung tissue was detected by RT-PCR. The results showed that WAt / Pbm, WAi / Pbm, WAm / Pbm, Wcol / Pbm and mucus secretion area in asthma model group, MT group and DXM group were both higher than those in normal control group and asthma group (P <0.05). There was no significant difference between MT group and DXM group (P> 0.05). The levels of TGF-βmRNA in asthmatic group, MT group and DXM group were significantly higher than those in normal control group and asthma group (P <0.01). There was no significant difference between MT group and DXM group (P> 0.05). The above shows that MT can reduce the level of TGF-βmRNA in the lung tissue of asthmatic mice and partly suppress the airway remodeling, which regulates the effect of dexamethasone.