论文部分内容阅读
目的:建立利心丸含量测定方法。方法:采用高效液相色谱(HPLC)法测定。色谱柱:Zorbax SBAq(5μm,4.6mm×250mm),流动相:甲醇-乙腈-水-二乙胺(52∶20∶28∶0.08);检测波长:282nm,柱温:25℃,流速:1.0m L/min。结果:粉防己碱和防己诺林碱的线性范围分别是0.2580-1.5480μg(r=0.9996)、0.1348-0.8085μg(r=0.9998),粉防己碱和防己诺林碱的平均加样回收率分别是97.88%(RSD=1.61%)、98.18%(RSD=2.08%)。结论:该方法操作简便易行,专属性强,准确可靠,可用于利心丸的质量控制。
Objective: To establish the method of determination of Xixin Pills. Methods: The determination by high performance liquid chromatography (HPLC). Column: Zorbax SBAq (5μm, 4.6mm × 250mm), mobile phase: methanol-acetonitrile-water- diethylamine (52:20:28:08); detection wavelength: 282nm; m L / min. RESULTS: The linear ranges of tetrandrine and nimesulide were 0.2580-1.5480μg (r = 0.9996) and 0.1348-0.8085μg (r = 0.9998), respectively. The mean recoveries of tetrandrine and nifedrine were Is 97.88% (RSD = 1.61%), 98.18% (RSD = 2.08%). Conclusion: The method is simple and easy to operate, with high specificity, accurate and reliable, and can be used for the quality control of Lixin Pills.