论文部分内容阅读
目的 探讨肾病综合征 (NS)儿童外周血单个核细胞 (PBMC)中活化蛋白 1(AP 1)和糖皮质激素受体 (GR)的DNA结合活性的变化 ,体外实验加入地塞米松 (DEX)以及泼尼松治疗后对二者的影响。方法 用凝胶电泳迁移率变化分析法 (EMSA)和同位素放射性自显影法分析了NS儿童PBMC中AP 1、GR的DNA结合活性。结果 (1)NS组基础状态AP 1DNA活性为 2 6 4 1(0 6～ 35 1 8) ,对照组为 0 8(0～ 1 1) ,两组比较差异有非常显著意义 (Z =2 72 ,P <0 0 1) ;佛波酯刺激状态下AP 1DNA活性为 5 14 8(1 1～ 6 16 7) ,对照组为 1 1(0～ 36 6 ) ,两组比较差异有非常显著意义 (Z =2 6 4,P <0 0 1) ;经DEX作用后NS组AP 1的DNA活性与对照组比较 ,差异无显著意义 (Z =0 6 4,P >0 0 5 )。 (2 )NS组基础状态GR的DNA活性为 7 6 (0 2～ 11 7) ,对照组为 45 2 (8 1～ 95 2 ) ,两组比较差异有显著意义(Z =2 5 6 ,P <0 0 5 ) ;TPA刺激状态GR的DNA活性为 5 3(0 0～ 10 7) ,对照组为 78 1(7 8～ 97 4) ,两组比较差异有显著意义 (Z =2 40 ,P <0 0 5 ) ,经DEX作用后GR的DNA活性与对照组比较 ,差异无显著意义 (Z =0 96 ,P >0 0 5 )。 (3)经泼尼松治疗尿蛋白转阴 1周后 ,AP 1DNA活性为 1 0 5 (0 95～1 44 ) ,与治疗前 [2 87(1
Objective To investigate the changes of DNA binding activity of activated protein 1 (AP 1) and glucocorticoid receptor (GR) in peripheral blood mononuclear cells (PBMCs) of children with nephrotic syndrome (NS). Dexamethasone (DEX) And the effect of prednisone on both. Methods DNA binding activity of AP 1 and GR in PBMCs of NS children was analyzed by EMSA and isotope autoradiography. Results (1) The activity of AP 1DNA in NS group was 2641 (0 6 ~ 35 1 8) and 0 8 (0 ~ 1 1) in control group. There was significant difference between the two groups (Z = 2 72 , P <0.01). The AP 1 DNA activity of phorbol ester-stimulated cells was 51 14 8 (1 1 ~ 6 16 7) and that of the control group was 1 1 (0 ~ 36 6). There was significant difference between the two groups (Z = 2644, P <0.01). There was no significant difference in the activity of DNA of AP1 in NS group after DEX treatment compared with the control group (Z = 0 64, P 0 05). (2) The DNA activity of basic GR in NS group was 76 (0 2 ~ 11 7) and 45 2 (8 1 ~ 95 2) in control group. There was significant difference between the two groups (Z = 256, P <0 0 5). The DNA activity of GR in the stimulated state of TPA was 53 (0 0 ~ 107) and 78 1 (78 ~ 97 4) in the control group, with significant difference between the two groups (Z = 2 40, P <0 05). The DNA activity of GR after DEX treatment was not significantly different from that of the control group (Z = 0 96, P 0 05). (3) After treated with prednisone for 1 week, the activity of AP 1DNA was 100% (0 95 ~ 1 44)