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目的 探讨长期深低温冻存脐血的移植活性及干细胞在裸鼠体内的迁移规律。方法 选取 30只BALB/Cnu+裸鼠 ,随机分为 3组。经致死量放射线照射后分别给予裸鼠人冻存后脐血单个核细胞、新鲜脐血以及生理盐水外周静脉输入 ,观察并比较小鼠存活情况和移植存活证据 ,采用PCR法及流式细胞术检测干细胞在小鼠体内的迁移、定居情况。结果 冻存脐血组小鼠存活率及存活时间均显著高于生理盐水组 ,而与新鲜脐血组无显著性差异 (P >0 .0 5 ) ;PCR法及流式细胞术检测小鼠体内人特异性基因及细胞均证实人冻存脐血已在小鼠骨髓内定居并植入存活 ,持续至少 1 30d ;但冻存脐血组干细胞的迁移情况与新鲜脐血组有所差异 ,裸鼠的肝和肾不易出现人特异性基因。结论 冻存可长期保存干细胞活性 ,但可能影响干细胞表面定居相关粘附分子 ,从而影响其迁移行为
Objective To investigate the transplantion activity of cord blood and the migration of stem cells in nude mice after long-term cryopreservation. Methods Thirty BALB / Cnu + nude mice were randomly divided into three groups. After lethal dose of radiation, nude mice were given umbilical cord blood mononuclear cells after cryopreservation, fresh umbilical cord blood and saline intravenous infusion were observed and compared survival and survival of mice survival evidence, PCR and flow cytometry Detection of stem cell migration in mice, colonization. Results The survival rate and survival time of cryopreserved cord blood group were significantly higher than that of saline group, but no significant difference with fresh cord blood group (P> 0.05). PCR and flow cytometry In vivo human-specific genes and cells were confirmed in human frozen umbilical cord blood has been settled and implanted in mice bone marrow survival for at least 130d; but the survival of frozen cord blood stem cell migration and fresh cord blood group differences, Nude mice liver and kidney is not prone to human-specific genes. Conclusion Cryopreservation can preserve the activity of stem cells for a long time, but may affect the adhesion molecules on the surface of stem cells and affect the migration behavior