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为明确山东省露地辣椒主产区炭疽病致病菌的种类,评价不同药剂对致病菌的毒力和活体接种防效,筛选高效防治药剂,山东农业大学植物保护学院研究人员从菏泽、济宁等5个辣椒主产区采集感炭疽病的辣椒果实,经分离、纯化培养后,观察菌落及分生孢子的形态、大小和病原菌的致病性,利用真菌通用引物ITS4和ITS5对4个典型代表菌株的rDNA-ITS进行扩增,对扩增
In order to clarify the types of anthracnose pathogenic bacteria in the peppers and peppers of Shandong Province, and to evaluate the virulence of different agents against pathogenic bacteria and the inoculation efficiency of inoculum, the effective screening and prevention agents were screened. The researchers from Shandong Agricultural University, Five pepper producing areas were collected for the anthracnose-infected pepper fruits. After isolated, purified and cultured, the morphology and size of the colonies and conidia and the pathogenicity of the pathogenic bacteria were observed. Four common fungal primers, ITS4 and ITS5, Representative strains of rDNA-ITS amplification, amplification