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建立了生物素标记探针技术，以便用于戊型肝炎病毒（ｈｅｐａｔｉｔｉｓＥｖｉｒｕｓ，ＨＥＶ）的诊断及与肠道病毒的鉴别。用Ａ５４９细胞培养ＨＥＶ８７Ａ株，收集上清，浓缩沉淀，酚／氯仿抽提ＲＮＡ，ＲＴ－ＰＣＫ扩增ｃＣＮＡ，将其直接点于硝酸纤维素膜或扩增产物经琼脂糖凝胶电泳分离后，转移到硝酸纤维素膜上，制备待检样品。以光敏生物素标记ＨＥＶＥＴ１．１４１８６ｐ探针，对硝酸纤维素膜进行点杂交和Ｓｏｕｔｈｅｒｎ印迹杂交。结果表明，制备的ＨＥＶ探针，特别是合成的寡核苷酸ＨＥＶ探针和肠道病毒探针，能够分别特异地与８７Ａ株病毒和肠道病毒Ｅｃｈｏ－１３型病毒的核酸杂交。该方法特异性强，较单独用ＰＣＲ扩增、经琼脂糖凝胶电泳敏感性高，特异性强，且结果能长期保存，可用于戊型肝炎的临床诊断。
A biotin-labeled probe was developed for the diagnosis of hepatitis E virus (HEV) and its identification with enterovirus. The HEV87A strain was cultured with A549 cells, the supernatant was collected, the precipitate was precipitated, the RNA was extracted with phenol / chloroform, and the cCNA was amplified by RT-PCK. The cRNA was directly spotted on the nitrocellulose membrane or amplified by agarose gel electrophoresis. Transferred to a nitrocellulose membrane to prepare samples to be tested. The HEVET1.14186p probe was labeled with photobiotin and spotted and Southern blot hybridized to the nitrocellulose membrane. The results show that the prepared HEV probes, particularly the synthetic oligonucleotide HEV probe and the enterovirus probe, are capable of specifically hybridizing with the 87A strain of virus and the Enterovirus Echo-13 type of virus respectively. The method is more specific and more rapid than PCR alone. It has high sensitivity and specificity by agarose gel electrophoresis and can be preserved for a long time. It can be used for the clinical diagnosis of hepatitis E.