目的建立HPLC法测定紫草中左旋紫草素含量的方法。方法采用Shimpack C18柱(4.6×250 mm,5μm),甲醇:0.1%磷酸溶液(85:15)为流动相,流速为1.0 m L·min-1,柱温:25℃,检测波长:515 nm。结果左旋紫草素浓度在5.3~106μg·m L-1之间与峰面积线性关系良好,r=0.999,8;平均回收率为99.5%,RSD为1.98%。结论本法操作简便,重复性好,可用于紫草中左旋紫草素的质量控制。 Objective To establish a HPLC method for the determination of L-shikonin in Lithospermum. Methods The mobile phase consisted of a mobile phase of Shimpack C18 column (4.6 × 250 mm, 5μm) and methanol: 0.1% phosphoric acid (85:15) at a flow rate of 1.0 m L · min-1. The detection wavelength was 515 nm . Results The concentration of L-shikonin in the range of 5.3 ~ 106μg · m L-1 was linear with the peak area, r = 0.999,8; the average recovery was 99.5% and the RSD was 1.98%. Conclusion This method is simple, reproducible and can be used for the quality control of Lithospermum in Lithospermum.