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运用序列特异性寡核苷酸探针23个,其中DQA_19个,DQB_114个,对48例IDDM及46例正常对照的白细胞DNA PCR扩增产物进行斑点杂交,检验DQA_1及DQB_1等位基因。发现HLA-DQ_α_(52~-)精氨酸及DQβ_(57~-)非天门冬氨酸的频率在IDDM中显著增高,与IDDM易感性高度相关。DQβ_(57~-)天门冬氨酸在IDDM中显著减少,似对IDDM的发病有“抵抗”作用。与白种人相比,NA/NA纯合子在IDDM中的频率显著低于白种人。A/A纯合子的频率又显著高于白种人。因此,DQ一个氨基酸的改变只是华人IDDM易感性的一个重要相关因素,不是唯一主要因素。
Twenty - three sequence - specific oligonucleotide probes, DQA_19 and DQB_114, were used to detect the DQA_1 and DQB_1 alleles by dot blot hybridization on 48 white blood cells from IDDM and 46 normal controls. The frequencies of HLA-DQ_α_ (52 ~ -) arginine and DQβ_ (57 ~ -) non-aspartate were found to be significantly increased in IDDM and highly correlated with IDDM susceptibility. DQβ_ (57 ~ -) aspartate significantly decreased in IDDM, which seems to be “resistant” to the pathogenesis of IDDM. Compared with Caucasians, NA / NA homozygotes had a significantly lower frequency in IDDM than Caucasians. The frequency of A / A homozygotes was significantly higher than that of Caucasians. Therefore, an alteration of one amino acid in DQ is only an important correlation factor of Chinese IDDM susceptibility, not the only major factor.