冻融胚胎复苏状态对移植周期临床结果的影响

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目的 观察冻融胚胎复苏状态对移植周期临床结果的影响。 方法  94对夫妇共进行冻融胚胎移植 (F ET) 99周期。其中自然周期 67个 ,激素替代治疗 3 2周期。冻融 2d或 3d胚胎采用慢速冷冻法和快速解冻法 ,根据卵裂球存活程度分为 3组 :A组为全部移植 1 0 0 %卵裂球存活胚胎 ;B组为全部移植部分卵裂球受损胚胎和C组为混合移植。 结果  99个周期共融解胚胎 3 3 0个、移植 2 87个 ,融胎存活率 87% ,临床妊娠率 2 3 % ( 2 3 / 99) ,胚胎种植率 1 0 % ( 3 0 / 2 87)。A组临床妊娠率 3 1 % ( 6/ 1 4 )、种植率1 8% ( 1 0 / 3 8) ,B组分别为 6% ( 1 / 1 6)及 2 % ( 1 / 41 ) ,C组分别为 2 3 % ( 1 6/ 69)及 9% ( 1 9/2 0 8) ,A ,B两组比较差异有显著意义 (P <0 .0 5)。进一步分析F ET胚胎中含 4或 8细胞卵裂球完好胚胎数 0 2 ,≥ 3的胚胎种植率 ,分别为 4.5%、1 0 %、1 2 .5%及 43 % ;4组间差别有显著性 (P <0 .0 5)。 结论 移植冻融胚胎卵裂球复苏状态与临床妊娠率密切相关 Objective To observe the effect of frozen-thawed embryo resuscitation on the clinical outcome of transplantation. Methods Ninety-four couples underwent 99 cycles of frozen-thawed embryo transfer (F ET). Of which 67 natural cycles, hormone replacement therapy 3 2 cycles. Frozen and thawed 2d or 3d embryos were divided into 3 groups according to the survival rate of blastomeres by the method of slow freezing and rapid thawing: group A was 100% blastomeres surviving all transplanted embryos; group B was all partial cleavage Damaged embryos and group C were mixed grafts. Results Three hundred and seventy embryos were reconstructed in 99 cycles, with 2 87 transplants. The survival rate of fusion was 87%, the clinical pregnancy rate was 23% (23/99) and the embryo implantation rate was 10% (3/87) . In group A, the clinical pregnancy rate was 31% (6/1 4), the implantation rate was 18% (10/38) and that of group B was 6% (1/16) and 2% (1/41), respectively The difference was significant between groups A and B (P <0.05). The differences were significant between groups A and B (23% (16/69) and 9% (19/280, respectively. Further analysis showed that the embryo implantation rate of embryos with 4 or 8 blastomeres with 0 2, 3 embryos in F ET embryos were 4.5%, 10%, 12.5% ​​and 43%, respectively; the differences among the 4 groups were Significance (P <0.05). Conclusion The status of blastocyst resuscitation in frozen-thawed embryos is closely related to clinical pregnancy rate
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