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目的 探讨局部肾素 -血管紧张素系统 (RAS)在模拟失重所致血管分化性适应中的作用。 方法 大鼠通过尾部悬吊模拟失重 ,悬吊 4周后 ,处死大鼠 ,分别取基底动脉、颈总动脉、腹主动脉和股动脉组织 ,提取总核糖核酸 (RNA) ,用逆转录酶 -多聚酶链式反应 (RT- PCR) ,半定量检测各动脉组织局部 RAS关键成份血管紧张素原 (AGT)和血管紧张素转化酶 (ACE) m RNA的表达水平 ,并通过脱氧核糖核酸 (DNA)序列测定对 PCR结果进行验证。 结果 正常大鼠 4种不同部位的动脉组织均有 AGT和 ACE基因表达 ;4周模拟失重后 ,悬吊组大鼠基底动脉 AGT和 ACE m RNA水平明显升高 (P<0 .0 5 ) ,股动脉 AGT m RNA水平下降 (P<0 .0 5 ) ,其余两种血管组织则未见上述基因表达的明显变化。 结论 生理条件下的大鼠基底动脉、颈总动脉、腹主动脉和股动脉均有局部 RAS关键成份的表达 ;模拟失重后基底动脉和股动脉局部 RAS表达出现了性质相反的变化 ,提示局部RAS很可能参与了模拟失重后动脉血管结构和功能的重塑。
Objective To investigate the role of local renin-angiotensin system (RAS) in the adaptation to vascular differentiation induced by simulated weightlessness. Methods Rats were subjected to tail suspension to simulate weightlessness. After being suspended for 4 weeks, rats were sacrificed, and total RNA was extracted from the basilar artery, common carotid artery, abdominal aorta and femoral artery. The total RNA was extracted by reverse transcriptase - The expression of angiotensinogen (AGT) and angiotensin-converting enzyme (ACE) mRNA, a key component of local RAS in arterial tissues, was detected by semi-quantitative polymerase chain reaction (RT- Sequence analysis confirmed the PCR results. Results AGT and ACE mRNA expression were detected in all the four groups of normal rats. After 4 weeks of simulated weightlessness, the levels of AGT and ACE m RNA in the basilar artery of the suspension group were significantly increased (P <0.05) Femoral artery AGT m RNA levels decreased (P <0. 05), the other two vascular tissues did not see the significant changes in gene expression. Conclusions The basal artery, common carotid artery, abdominal aorta and femoral artery of rats under physiological condition have the expression of the key components of RAS. The changes of RAS in the basilar artery and femoral artery after simulated weightlessness show the opposite changes in nature, suggesting that local RAS It is likely involved in the remodeling of arterial structure and function after simulated weightlessness.