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目的对北京市丰台区某小学2014年5-6月间首起GⅡ.8诺如病毒感染性肠胃炎暴发疫情进行病原体鉴定及基因分型,为防控提供科学依据。方法采用实时荧光定量PCR法对12份病例标本进行诺如病毒核酸检测,阳性标本经RT-PCR扩增RNA多聚酶区部分序列,使用Bio Edit及Mega5.2.2软件对扩增序列进行同源性及进化分析。结果20件疫情标本检出12件诺如病毒核酸阳性,阳性率60.00%,序列分析表明为GⅡ.8型。结论此次诺如病毒感染性腹泻暴发疫情可能是由于学生间接触传播导致,是丰台区首次发现由GⅡ.8型诺如病毒引起的急性胃肠炎暴发疫情,需要加强监测。
Objective To identify pathogen and genotype the first GⅡ.8 Norovirus Infectious Gastritis outbreak in a primary school in Fengtai District of Beijing from May to June 2014, providing a scientific basis for prevention and control. Methods Real-time fluorescence quantitative PCR was used to detect norovirus nucleotides in 12 cases. The positive samples were amplified by RT-PCR and sequenced using Bio Edit and Mega 5.2.2 software. Evolutionary analysis. Results Of the 20 outbreak samples, 12 cases of Norovirus positive were detected, the positive rate was 60.00%. The sequence analysis indicated that they were G Ⅱ. Conclusions The outbreak of Norovirus infectious diarrhea may be caused by the contact transmission among students. It is the first case of outbreak of acute gastroenteritis caused by Norovirus Ⅱ GⅡ.8 in Fengtai District. It is necessary to strengthen the surveillance.