全反式维甲酸对球囊损伤大鼠胸主动脉VSMC表型变化及增殖的影响

来源 :青岛大学医学院学报 | 被引量 : 0次 | 上传用户:ab869
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
①目的探讨全反式维甲酸(ATRA)对球囊剥脱大鼠胸主动脉内皮后血管平滑肌细胞(VSMC)表型变化及增殖的影响。②方法雄性SD大鼠54只,随机分为3组。ATRA治疗组(n=24)行球囊剥脱大鼠胸主动脉内皮术,术前4d开始予ATRA芝麻油混悬液(30mg.kg-1.d-1)灌胃至术后处死;手术组(n=24)行球囊剥脱大鼠胸主动脉内皮术,术前4d开始予芝麻油(1mL.kg-1.d-1)灌胃至术后处死;对照组(n=6)除不插入球囊导管外,其他操作同手术组。分别于术后2、7、14、28d取胸主动脉应用苏木精-伊红染色、免疫组化和计算机图像分析法进行形态学、平滑肌α肌动蛋白(SM--αactin)和增殖细胞核抗原(PCNA)表达水平检测。③结果正常动脉VSMC高表达SM--αactin,处于非增殖状态;损伤后中膜SM--αactin表达迅速下降,2d达最低水平,而增殖旺盛,后趋向正常水平;新生内膜在术后7d出现,处于高度增殖状态,SM--αactin含量少,术后14、28d的SM--αactin表达明显增多,而增殖下降。ATRA治疗能明显抑制中膜SM--αactin的下调,诱导内膜和中膜SM--αactin表达,显著抑制VSMC的迁移和增殖,明显抑制新生内膜形成和管腔缩窄。SM--αactin表达与PCNA表达呈显著负相关(r=-0.738,P<0.01)。④结论维持并诱导VSMC于较高的分化状态,抑制VSMC迁移和增殖,是ATRA抑制球囊损伤大鼠主动脉后新生内膜增生和管腔狭窄的可能机制之一。 Objective To investigate the effect of all-trans retinoic acid (ATRA) on the phenotype and proliferation of vascular smooth muscle cells (VSMCs) in the aorta of balloon-evacuated rats. Methods 54 male SD rats were randomly divided into three groups. ATRA treatment group (n = 24) underwent balloon-stripped thoracic aorta endothelia. ATRA sesame oil suspension (30mg.kg-1.d-1) (n = 24) underwent balloon-stripped thoracic aortic endothelium. Sesame oil (1 mL.kg-1.d-1) Insert the balloon catheter, other operations with the surgery group. The thoracic aorta was taken at 2, 7, 14 and 28 days after operation. The morphology, smooth muscle actinine (α - actin) and proliferating cell nuclear were detected by hematoxylin - eosin staining, immunohistochemistry and computer image analysis. Antigen (PCNA) expression level detection. Results The expression of SM - αactin in normal artery VSMC was highly nonsproliferative. The expression of SM - αactin in the mesangial membrane rapidly decreased after 2 days, reached the lowest level on the 2nd day, and proliferated vigorously and then returned to the normal level. The neointimal hyperplasia was found on the 7th day Appeared in a highly proliferative state, the content of SM - αactin was small, the expression of SM - αactin increased obviously and the proliferation decreased after 14 and 28 days. ATRA treatment could significantly inhibit the down - regulation of SM - αactin, induce the expression of SM - αactin in intima and media, significantly inhibit the migration and proliferation of VSMC, and significantly inhibit neointimal formation and lumen narrowing. There was a significant negative correlation between SM - αactin expression and PCNA expression (r = -0.738, P <0.01). ④Conclusion Maintaining and inducing VSMCs to differentiate into higher states and inhibiting the migration and proliferation of VSMCs is one of the possible mechanisms by which ATRA can inhibit neointimal hyperplasia and stenosis after aorta injury in balloon-injured rats.
其他文献
乳腺癌除手术切除外,迄今尚无满意的治疗措施,但手术根治切除率低。自杀基因(suicidegene)疗法的出现,为乳腺癌的治疗提供了一种较为有效和具有临床应用潜力的治疗策略。本文
Kallmann综合征(KS)又称卡尔曼综合征,或性幼稚嗅觉丧失综合征,是一种先天性促性腺功能低下和嗅觉缺失联合出现的病征。发病率男性为1/10000,女性为1/50000。患者一般青春晚
葡萄糖一直被认为是脑内氧化代谢供能的唯一物质,乳酸则是脑细胞无氧糖酵解产生的代谢废物。最近有人提出星形胶质细胞-神经元乳酸穿梭假说,指出乳酸可作为一种重要的能量底
丝裂原活化蛋白激酶(MAPK)信号通路广泛存在于哺乳动物细胞中,主要由MAPKKK、MAPKK和MAPK 3类保守的蛋白激酶组成,与多种细胞反应(如细胞增殖、分化、转化及凋亡等)及机体多
目的:研究过氧化物酶增殖因子激活受体γ(peroxisomeproliferatorsactivatedreceptorγ,PPARγ)激活剂罗格列酮对人胃癌细胞系MKN45增殖和MMP7基因表达的影响。方法:不同浓度
根据Genbank中已发表的犬属Hepcidin序列(AY772532),设计一对特异性引物,采用RT-PCR技术,从犬的肝脏中扩增出长258bp的基因.经测序表明该基因序列为犬属Hepcidin序列,对其序
目的:观察极化液(葡萄糖-胰岛素-钾液,G IK)对急性心肌缺血/再灌注(M I/R)犬心脏功能、冠脉血流量及心肌损伤的影响,分析胰岛素在G IK上述效应中的作用。方法:制备犬M I/R模
Bcl-2、Bax是调控细胞凋亡的结构相似、作用相反的一对胞内蛋白质,二者的比例是决定对细胞抑制作用强弱的关键因素.Bcl-2/Bax与镉性肾细胞凋亡的发生、发展及转归密切相关.本
主要从内皮损伤的分子病理角度综述高同型半胱氨酸血症导致动脉硬化的可能机制。 Mainly from the molecular pathology of endothelial injury overview of homocysteine
FOXP3是一个与调节性T细胞的生成及功能有关的转录因子.在小鼠体内,仅表达于CD4+CD25+T细胞,在人类则不仅可表达于CD4+CD25+T细胞,还可表达于CD8+CD28-T细胞,但以前者为主.胸