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目的:研究螺旋藻激酶(SPK)对动脉粥样硬化模型大鼠血管内皮功能的影响。方法:将60只大鼠随机分为正常对照组(蒸馏水)、模型组(蒸馏水)、阳性对照组(辛伐他汀,0.005 g/kg)和SPK低、中、高剂量组(80、160、320 U/kg)。除正常对照组外,其余各组大鼠均复制动脉粥样硬化模型;同时,各组大鼠ig相应药物,每天1次,连续给药12周。测定大鼠血清中总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)的含量;苏木精-伊红染色观察大鼠胸主动脉血管内膜的形态改变。结果:与正常对照组比较,模型组大鼠血清中TC、TG、LDL-C、IL-6和TNF-α含量增加(P<0.01),HDL-C含量降低(P<0.01);血管内皮细胞脱落,内膜增生、向管腔内突起,平滑肌细胞增殖且排列紊乱,中膜弹力纤维崩解断裂。与模型组比较,各给药组大鼠血清中TC、TG、LDL-C、IL-6和TNF-α含量下降(P<0.05或P<0.01),阳性对照组和SPK中、高剂量组大鼠血清中HDL-C含量增加(P<0.05);给药组大鼠血管内皮细胞形态较模型组明显改善,其中SPK中、高剂量组大鼠血管内皮细胞各层结构完整、内膜基本光滑,SPK中剂量组大鼠血管中膜平滑肌细胞排列稍紊乱,与正常对照组比较无明显变化。结论:SPK有明显的降脂和抗炎作用,可保护血管内皮功能;其作用机制可能与降低血清中TC、TG、LDL-C、IL-6、TNF-α含量和升高血清中HDL-C含量有关。
AIM: To investigate the effects of spirulina kinase (SPK) on vascular endothelial function in atherosclerotic rats. Methods: Sixty rats were randomly divided into normal control group (distilled water), model group (distilled water), positive control group (simvastatin, 0.005 g / kg) and SPK low, middle and high dose group 320 U / kg). In addition to the normal control group, the rest of the rats were replicated atherosclerosis model; the same time, each group of rats ig appropriate drugs, once a day, continuous administration for 12 weeks. The levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), interleukin 6 (IL-6) Tumor necrosis factor α (TNF-α) content; thymus-eosin staining rat thoracic aorta vascular intimal morphological changes. Results: The contents of TC, TG, LDL-C, IL-6 and TNF-α in the model group were significantly higher than those in the normal control group (P <0.01) Cells shedding, intimal hyperplasia, protruding to the lumen, smooth muscle cell proliferation and dislocation, disintegration of the disintegration of the middle membrane elastic fibers. Compared with the model group, the content of TC, TG, LDL-C, IL-6 and TNF-αin the serum of each administration group decreased (P <0.05 or P <0.01) The content of HDL-C in rat serum increased (P <0.05). The morphology of vascular endothelial cells in the treated group was significantly improved compared with the model group, and the levels of vascular endothelial cells in SPK medium and high dose groups were intact, Smooth, mid-dose SPK rat vascular smooth muscle cells arranged slightly disorder, compared with the normal control group no significant change. Conclusion: SPK has obvious lipid-lowering and anti-inflammatory effects and can protect endothelial function. Its mechanism may be related to the decrease of serum TC, TG, LDL-C, IL-6 and TNF- C content.