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背景:成牙骨质细胞在牙根形成、牙周病牙骨质的再生以及正畸牙根吸收的修复中起着重要的作用,其在体外培养状态下对力学信号反应特性的变化,在国内外少见报道。目的:分析正畸牙移动张力侧及压力侧两种不同受力形式与牙骨质改建的关系。方法:采用四点弯曲细胞力学加载仪,对体外培养的成牙骨质样细胞OCCM30分别施加2000με张、压应力,应用流式细胞术检测应力刺激3,6,12,24h后OCCM30细胞周期及增殖指数的变化。结果与结论:2000με张、压应力加载3,6h后,成牙骨质样细胞OCCM30在S期的细胞百分率明显减小,细胞增殖活性降低;随着加力时间的延长,S期细胞百分率回升,加载24h后S期细胞百分率与对照组无明显差异,细胞增殖活性恢复。张、压应力对增殖活性的影响趋势一致,相互之间差别无显著性意义。
BACKGROUND: Calcified cementoblasts play an important role in root formation, periodontal bone cement remodeling and orthodontic tooth root resorption. The changes of mechanical signaling response under in vitro culture conditions have been reported both at home and abroad Rarely reported. OBJECTIVE: To analyze the relationship between two different forms of stress and cementodialysis on the tension side and pressure side of orthodontic tooth movement. Methods: Four-point bending cell mechanics loader was used to measure the cell cycle of OCCM30 in vitro and to investigate the effect of stress on the cell cycle of OCCM30 cells after stress stimulation at 3, 6, 12 and 24 hours by flow cytometry Proliferation index changes. RESULTS AND CONCLUSION: The cell percentage of OCCM30 in S phase was significantly decreased and cell proliferation activity was decreased after 2000με sheet and 3,6h of compressive stress loading. With the increase of time, the percentage of S phase cells rose After 24 hours of loading, the percentage of S phase cells did not change significantly with the control group, and the cell proliferation activity recovered. Zhang, compressive stress on the proliferation of the trend of the same, no significant difference between each other.