Objective: The aim of this study was to analyze the expression of CC chemokine receptor 7 (CCR7) in pulmonary tumor tissue and metastasized lymph nodes of non-small cell lung cancer (NSCLC), explore the relationship between the expressions of CCR7 in pulmonary tumor tissue and metastasized lymph nodes, and discuss the significance. Methods: SABC immunohistochemical staining was used to investigate the expression of CCR7 by rabbit anti-human CCR7 mono-clonal antibody, and the specimens were 17 cases of adenocarcinoma, 17 cases of squamous cell carcinoma, 12 cases of adenosquamous carcinoma, 4 cases of large cell carcinoma and 28 cases of metastasized lymph nodes of lung cancer. Negative control sections used 5 cases of inflammatory pseudotumor and 20 cases of normal lung tissue. Two independent pathologists observed all the specimens in the high power field (× 400) of microscope by double blind method. Results: (1) The expression of CCR7 in pulmonary tumor tissue was stronger than normal lung tissue (P < 0.005); (2) The expressions of CCR7 in pulmonary tumor tissues and metastasized lymph nodes had no significant differences (P = 0.177); (3) The expression of CCR7 had correlation with lymph nodes metastasis, and the expression in lymph nodes metastasis group was more than that of no lymph nodes metastasis group (P = 0.016); (4) Along with the increment of clinical stage, the CCR7 expression had a tendency to increase (P = 0.003). Conclusion: CCR7 has rich expression in carcinoma cell nests and lymph node metastasis. It demonstrates that CCR7 may be related to the development of lymph node metastasis in NSCLC. Objective: The aim of this study was to analyze the expression of CC chemokine receptor 7 (CCR7) in pulmonary tumor tissue and metastasized lymph nodes of non-small cell lung cancer (NSCLC), explore the relationship between the expressions of CCR7 in pulmonary tumor Tissue and metastasized lymph nodes, and discuss the significance. Methods: SABC immunohistochemical staining was used to investigate the expression of CCR7 by rabbit anti-human CCR7 mono-clonal antibody, and the specimens were 17 cases of adenocarcinoma, 17 cases of squamous cell carcinoma , 12 cases of adenosquamous carcinoma, 4 cases of large cell carcinoma and 28 cases of metastasized lymph nodes of lung cancer. Negative control sections used 5 cases of inflammatory pseudotumor and 20 cases of normal lung tissue. Two independent pathologists observed all the specimens in the high power field (× 400) of microscope by double blind method. Results: (1) The expression of CCR7 in pulmonary tumor tissue was stronger than norm (2) The expressions of CCR7 in pulmonary tumor tissues and metastasized lymph nodes had no significant differences (P = 0.177); (3) The expression of CCR7 had correlation with lymph node metastasis, and the expression in lymph nodes metastasis group was more than that of no lymph node metastasis group (P = 0.016); (4) Along with the increment of clinical stage, the CCR7 expression had a tendency to increase has rich expression in carcinoma cell nests and lymph node metastasis. It demonstrates that CCR7 may be related to the development of lymph node metastasis in NSCLC.