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北京口岸从英国邮寄进境的大岩桐(Sinningia speciosa Benth)宿根植物根际介质中分离到1种孢囊线虫属的2龄幼虫,通过形态鉴定和分子特征分析,鉴定其为三叶草孢囊线虫(Heterodera trifolii Goffart,1932)。该英国群体的2龄幼虫体长439~539μm;唇区有3或4个唇环;口针粗壮,长26.3~29.7μm,基部球圆形或前端略凹陷;侧线4条;尾长圆锥形,末端细圆,透明尾长27.7~39.5μm,约占尾长的60%。基于核糖体DNA的28S区和ITS区序列构建的系统发育树证实该鉴定结果。
Beijing Port The 2nd instar larvae of the genus Cyclospora were isolated from rhizosphere of rhizosphere of Sinningia speciosa Benth mailed into the United Kingdom and identified as clover cyst nematode Heterodera trifolii Goffart, 1932). The second instar larvae of the British population are 439-539 μm in length with three or four lip rings in the lip region; sturdy stitches, 26.3-29.7 μm in length, slightly rounded at the anterior or basal dome; lateral lines 4; caudal-long conical , The end of the round, transparent tail length 27.7 ~ 39.5μm, about 60% of tail length. The phylogenetic tree based on the sequences of 28S region and ITS region of ribosomal DNA confirmed the identification results.