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目的了解丙型肝炎病毒（ＨＣＶ）和人类免疫缺陷病毒Ｉ型（ＨＩＶ－１）混合感染标本中ＨＣＶ基因型情况。方法采用了根据ＨＣＶ５′端非转译区基因组所设计的反相探针杂交测定方法（ＩＮＮＯＬｉＰＡ，ＨＣＶⅡ），对来自六个地区的１７份抗－ＨＣＶ和抗－ＨＩＶ－１双阳性标本进行ＨＣＶ基因型分型研究。结果在１７份标本中，７份（４１．１８％）为Ｉ（１ｂ）型病毒，５份（２９．４１％）为Ⅱ（２ａ／２ｃ）型病毒，５份（２９．４１％）为Ｉ型（１ｂ）和Ⅱ（２ａ／２ｃ）混合型病毒。结论发现ＨＣＶ与ＨＩＶ－１合并感染标本中存在Ｉ（１ｂ）型、Ⅱ（２ａ／２ｃ）型以及Ｉ（１ｂ）型与Ⅱ（２ａ／２ｃ）型混合型ＨＣＶ。值得注意的是对α－干扰素治疗不敏感并且较易趋于导致严重的慢性肝炎、肝硬化和肝细胞癌的Ⅱ型ＨＣＶ感染的比率占７０．５８％（１２／１７）。
Objective To understand the HCV genotypes in mixed samples of hepatitis C virus (HCV) and human immunodeficiency virus type 1 (HIV-1). Methods A total of 17 anti-HCV and anti-HIV-1 double positive specimens from six regions were tested for HCV gene by reverse-phase probe hybridization assay (INNOLiPA, HCVⅡ) designed based on the 5 ’untranslated region Type Typing Study. Results Of the 17 specimens, 7 (41.18%) were type I (1b) viruses, 5 (29.41%) were type II (2a / 2c) viruses and 5 (29.41%) were Type I (1b) and II (2a / 2c) mixed viruses. CONCLUSION: I (1b), Ⅱ (2a / 2c) and I (1b) and Ⅱ (2a / 2c) mixed HCV exist in HCV and HIV-1 infected specimens. It is noteworthy that 70.58% (12/17) were insensitive to interferon-alpha therapy and were more likely to be predisposed to type II HCV infection leading to severe chronic hepatitis, cirrhosis and hepatocellular carcinoma.