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目的实验室测定褐家鼠对第二代抗凝血灭鼠剂大隆的敏感性,为抗药性监测提供本底参数。方法参照全国鼠类抗药性监测协作组统一方法[1],在实验室作无选择性摄食试验。将试鼠雌雄分开,随机分组,按4个不同食毒期摄食0.00012%大隆毒饵。用Bliss计算机程序,计算不同食毒期与对应死亡率之间关系。结果褐家鼠性别之间对大隆的敏感性没有显著性差异(Pb>0.05,PLFP50>0.05),故雌雄数据合并计算,得毒力回归方程y=3.62+5.00x,LFP50、LFP99及其95%置信限分别是1.89(1.61～2.23)和5.52(3.65～8.34)。结论按WHO修订以致死99%敏感靶标鼠种的食毒期(LFP99)取整天数作为抗药性检验标准,湛江地区褐家鼠对0.00012%的大隆毒饵食毒期超过6d存活为产生抗药性。
Objective To determine the sensitivity of Rattus norvegicus to Dalong, the second generation anticoagulant rodenticide, to provide background parameters for drug resistance monitoring. Methods According to the unified method of the national collaborative monitoring of drug resistance in rats [1], non-selective feeding test was conducted in the laboratory. Male and female rats will be separated, randomly divided into four different food poisoning period feeding 0.00012% Dalong bait. The Bliss computer program was used to calculate the relationship between different food poisoning and corresponding mortality. Results There was no significant difference (P> 0.05, PLFP50> 0.05) between the sexes of Rattus norvegicus for Dalong. Therefore, the male and female data were combined to calculate the virulence regression equation y = 3.62 + 5.00x, LFP50, LFP99 and their The 95% confidence limits are 1.89 (1.61 to 2.23) and 5.52 (3.65 to 8.34), respectively. Conclusion According to the revised WHO, lethal 99% of the sensitive target mice take the whole dietary period (LFP99) as the drug resistance test standard. In Zhanjiang area, Rattus norvegicus survived more than 6d for 0.00012% Medicinal properties.