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益气养血法是中医气血学说用于临床的重要治则,阐明其作用机理对临床及对中医气血本质的认识均有一定意义。在过去工作的基础上,我们采用放射性同位素示踪技术,分别以~3H-Leu、~3H-UR和~3H-TdR为参入前体,液闪测量,观察了益气养血法的代表方剂——当归补血汤对小鼠腹腔巨噬细胞(M_φ)DNA合成,对小鼠骨髓细胞蛋白质及RNA合成,对小鼠肝、脾,心和血清蛋白质合成的影响。
The method of supplementing Qi and nourishing blood is an important principle for the clinical application of traditional Chinese medicine theory of Qi and blood. It is of certain significance to clarify its mechanism of action and to understand the nature of blood and blood in TCM. On the basis of past work, we used radioactive isotope tracer technology, with ~3H-Leu, ~3H-UR, and ~3H-TdR as reference precursors, and liquid scintillation measurements to observe the representative formulas of the Qi and blood-enhancing methods. ——Effect of Danggui Buxue Decoction on synthesis of mouse peritoneal macrophage (M_φ) DNA, protein and RNA synthesis in mouse bone marrow cells, and protein synthesis in liver, spleen, heart and serum of mice.