对金属螯合色谱分离提取初乳免疫球蛋白和乳铁蛋白的新工艺进行了研究,并采用SDS-PAGE电泳分析法测定了分离得到的免疫球蛋白和乳铁蛋白的含量。试验结果表明,采用金属螯合色谱可较好地分离提取免疫球蛋白,其纯度可达89%。同时还可分离得到副产品乳铁蛋白,但含量较低,仅为33%。另外还对影响螯合色谱分离的因素进行了初步的探讨。 The new technology of separation and purification of colostrum immunoglobulin and lactoferrin by metal chelate chromatography was studied. The content of immunoglobulin and lactoferrin isolated were determined by SDS-PAGE electrophoresis. The results show that the metal chelate chromatography can be better separation and extraction of immunoglobulins, the purity of up to 89%. By-product lactoferrin was also isolated at the same time, but at a low level of 33%. In addition, the factors influencing the separation of chelate chromatography were also discussed.