论文部分内容阅读
本研究采用SSR分子标记方法,分析了36份制干辣椒品种资源间的遗传关系。从36对SSR标记中筛选出12对进行电泳分析,这12对引物扩增条带清晰且多态性丰富。分析结果表明:12对SSR引物共获得87条谱带,平均每对引物7.3条谱带,多态性位点58个,平均多态性比率66.67%;供试材料间的遗传相似系数介于0.277 8~0.805 6之间;遗传相似系数以0.76为阀值时,可将36个制干辣椒品种分为3大类群;进一步的群体结构分析表明3个类群间种质资源相互渗入情况非常明显,这可能是由于受到强烈的人工选择的影响。
In this study, SSR molecular markers were used to analyze the genetic relationship among 36 cultivars of pepper varieties. Twelve pairs of 36 pairs of SSR markers were screened for electrophoresis analysis. The 12 pairs of primers amplified bands with abundant polymorphism. The results showed that 87 bands were obtained from 12 pairs of SSR primers, with an average of 7.3 bands per primer and 58 polymorphic loci with an average ratio of 66.67%. The genetic similarity coefficients of the tested materials ranged from 0.277 8 ~ 0.805 6. When the genetic similarity coefficient was 0.76, the 36 cultivars of dried-off pepper varieties could be divided into three groups. Further analysis of population structure showed that the germplasm resources interpenetration between the three groups was very obvious This may be due to the strong artificial choice.