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目的:观察含PAcA/CTB的转基因番茄可食用防龋疫苗的免疫原性及免疫反应性。方法:将18只6~8周龄的雄性BALB/c小鼠,随机分为3组,每周分别灌胃含有PAcA/CTB嵌合蛋白的转基因番茄果汁(实验组)、非转基因番茄果汁(阴性对照组)、灭活全菌疫苗(阳性对照组)。于首次免疫前和免疫后第1、2、3、4周采集血液、唾液样品。ELISA检测其血清和唾液样本中的抗变异链球菌PAcA的IgG、IgA抗体效价。结果:阳性对照组和实验组小鼠血液和唾液中特异性抗体从免疫后1周开始升高,初始免疫后第4周达高峰,分别与阴性对照组比较存在统计学差异(P<0.05或P<0.01)。免疫后第2、3、4周阳性对照组与实验组差异有统计学意义(P<0.05)。结论:转基因番茄所表达的外源目的蛋白具有抗原性,能够诱导BALB/c小鼠产生免疫应答。
OBJECTIVE: To observe the immunogenicity and immunoreactivity of edible caries-preventive vaccine against transgenic tomato containing PAcA / CTB. Methods: Twenty-eight male BALB / c mice aged 6 to 8 weeks were randomly divided into three groups. The transgenic tomato juice (experimental group) containing the PAcA / CTB chimeric protein was intragastrically administrated every week. The non-transgenic tomato juice Negative control group), inactivated whole-cell vaccine (positive control group). Blood and saliva samples were collected before the first immunization and at 1, 2, 3 and 4 weeks after immunization. ELISA to detect IgG and IgA antibody titers against S. mutans PAcA in their serum and saliva samples. Results: The specific antibodies in the blood and saliva of the positive control group and the experimental group increased from 1 week after immunization and reached the peak at 4 weeks after the initial immunization, which were significantly different from the negative control group (P <0.05 or P <0.01). There was a significant difference between the positive control group and the experimental group at the 2nd, 3rd, 4th week after immunization (P <0.05). Conclusion: The exogenous protein expressed in transgenic tomato has antigenicity and can induce immune response in BALB / c mice.