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成纤维生长因子FGF1a(fibroblast growth factor 1a)是一类重要的生长因子,在细胞的发育过程中起着重要的作用。用实验室镜鲤EST数据库设计包含该基因CDS全长的特异引物,得到了FGF1a基因的全长CDS。经过克隆测序发现,镜鲤FGF1a基因全长的CDS包含444个碱基,其编码一个含有146个氨基酸的蛋白质,包含一个受体结合位点。与其它物种序列比对结果表明,镜鲤FGF1a蛋白与斑马鱼相似度最高,而与其亲缘关系较远的人类、老鼠、非洲爪蟾蜍及鸡相似度较低。根据所获得的基因序列设计探针,利用原位杂交技术发现FGF1a基因在鱼刚长鳞片的皮肤上表达较高,说明该基因可能参与鳞被发育;而同时在尾鳍、背鳍和腹鳍中也有特异的高表达信号,说明FGF1a可能也参与鳍条的发育过程。
Fibroblast growth factor 1a (FGF1a) is an important growth factor and plays an important role in the development of cells. The full-length CDS of FGF1a gene was obtained by designing a mirror library containing the full length CDS of the gene using the laboratory mirror carp EST database. After cloning and sequencing, the full-length CDS of the FGF1a gene in mirror carp contains 444 bases, which encodes a protein of 146 amino acids and contains a receptor binding site. The results of sequence alignment with other species showed that FGF1a protein of P. carp had the highest similarity with zebrafish, while its similarity with humans, mice, Xenopus laevis and chicken with lower genetic distance was lower. Based on the obtained gene sequences, probes were designed and the in situ hybridization technique was used to find that the FGF1a gene was highly expressed on the skin of fish scales, indicating that the gene may be involved in the development of scales. In the meanwhile, it is also specific in caudal, dorsal and pelvic fins Of the high expression signals, indicating that FGF1a may also participate in the development of the fin.