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RNA干扰(RNA interference,RNA i)是指细胞利用外源性或者内源性的双链小干扰RNA(sm all interfer-ing RNA,siRNA)激发相关的酶复合物对同源性mRNA进行切割、降解,从而在转录后水平阻断基因的表达,达到同源基因的减效表达或不表达。国内外细胞实验和动物实验均表明,在乙型肝炎病毒(hepatitis B virus,HBV)治疗中,RNA i由于具备特异、高效、无明显副作用等许多特点,较目前常用的干扰素和核苷类药物具有许多优势,在抗HBV治疗研究中展示了良好的应用前景。
RNA interference (RNAi) refers to the use of exogenous or endogenous double interfering RNA (sm all interfer-ing RNA, siRNA) to stimulate the relevant enzyme complex homologous mRNA cleavage, Degradation, thereby blocking the expression of the gene at the post-transcriptional level, to achieve the homologous gene reduced expression or not. Both cell and animal experiments both at home and abroad show that in the treatment of hepatitis B virus (HBV), RNAi has many characteristics such as specificity, high efficiency, no obvious side effects and so on. Compared with the currently used interferon and nucleosides Drugs have many advantages and show good application prospects in anti-HBV treatment research.