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广泛逆境胁迫蛋白(USPs)参与碳缺乏、缺氧、干旱和高盐等多种非生物胁迫,但在植物中的研究尚不深入。本文通过RACE-PCR的方法获得青杄PwUSP2基因的c DNA全长,共987bp,其中编码区723 bp,共编码240个氨基酸。利用生物信息学工具对其理化性质、二级结构和三级结构进行分析,结果显示,该蛋白理论分子质量为26.84 kDa,理论等电点为4.61,有丝氨酸和苏氨酸结合位点,为非跨膜的亲水蛋白。PwUSP2具有USP家族典型的Usp A结构域,但无典型的ATP结合位点G-2X-G-9X-G[S/T]。RT-qPCR分析表明,PwUSP2在青杄花粉、果实、种子、成熟叶、幼叶、成茎中均有表达,在果实中表达量较高。同时,PwUSP2在脱落酸(ABA)、茉莉酸甲酯(MeJA)等非生物胁迫下表达量有明显变化,推测PwUSP2可能参与青杄对逆境胁迫的响应。
Extensive stress stress proteins (USPs) are involved in a number of abiotic stresses such as carbon deficiency, anoxia, drought and salt stress, but studies in plants are not yet well established. In this study, the full length of c DNA of PwUSP2 gene was obtained from RACE-PCR using a total of 987bp, of which the coding region was 723 bp, encoding a total of 240 amino acids. Bioinformatics tools were used to analyze its physicochemical properties, secondary structure and tertiary structure. The results showed that the theoretical molecular mass of this protein was 26.84 kDa, the theoretical isoelectric point was 4.61, and the serine and threonine binding sites were Non-transmembrane hydrophilic protein. PwUSP2 has a Usp A domain typical of the USP family but no typical ATP binding site G-2X-G-9X-G [S / T]. RT-qPCR analysis showed that PwUSP2 was expressed in the pollens, fruits, seeds, mature leaves, young leaves and stems, and higher in fruits. At the same time, the expression level of PwUSP2 in abscisic acid (ABA) and methyl jasmonate (MeJA) significantly changed. It is speculated that PwUSP2 may be involved in the response of barley to stress.