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目的:通过探讨CD147和MMP-2在人脑胶质瘤中的表达及临床意义,为临床治疗提供参考。方法:选择2010年1月至2015年5月本院手术切除并经病理诊断确诊的脑胶质瘤标本70例作为观察组,根据WHO分型,将其分为低级别组31例和高级别组39例。另选取20例脑外伤患者并作内减压切除的标本作为对照组。采用免疫组化SP法检测CD147与MMP-2蛋白的表达,RT-PCR检测CD147mRNA表达,并探讨CD147表达与患者预后的关系。结果:观察组中CD147阳性表达率为75.71%,MMP-2阳性表达率74.29%,两者存在相关性(r=0.870,P<0.05)。高级别组和低级别组CD147mRNA表达水平均强于对照组,差异有统计学意义(均P<0.05),且高级别高于低级别组,差异有统计学意义(P<0.05)。复发患者CD147阳性表达率显著高于非复发患者,差异有统计学意义(P<0.05);生存时间<5年患者CD147阳性表达率显著高于生存时间≥5年患者,差异有统计学意义(P<0.05)。结论:人脑胶质瘤中CD147和MMP-2的表达与肿瘤恶性程度和患者预后密切相关,可为患者临床预后判断和临床治疗提供参考。
Objective: To provide a reference for clinical treatment by exploring the expression and clinical significance of CD147 and MMP-2 in human glioma. Methods: From January 2010 to May 2015, 70 patients with glioma surgically resected and pathologically diagnosed in our hospital were selected as the observation group. According to WHO classification, 31 cases were classified into low-grade group and high-grade Group of 39 cases. Another 20 cases of brain trauma patients and resection of the specimens as a control group. The expression of CD147 and MMP-2 protein was detected by immunohistochemical SP method. The expression of CD147 mRNA was detected by RT-PCR, and the relationship between the expression of CD147 and the prognosis was analyzed. Results: The positive rate of CD147 in the observation group was 75.71% and the positive rate of MMP-2 in the observation group was 74.29%. There was a correlation between them (r = 0.870, P <0.05). The expression levels of CD147 mRNA in high-grade group and low-grade group were significantly higher than those in control group (all P <0.05), and were higher in the high-grade group than in the low-grade group (P <0.05). The positive rate of CD147 in patients with recurrent disease was significantly higher than that in non-recurrent patients (P <0.05). The positive rate of CD147 in survival time <5 years was significantly higher than that in patients with survival ≥ 5 years P <0.05). Conclusion: The expression of CD147 and MMP-2 in human glioma is closely related to the degree of malignancy and the prognosis of patients. It may provide a reference for clinical judgment and clinical treatment.